A cell spot microarray method for production of high density siRNA transfection microarrays.

ABSTRACT:

Background

High-throughput RNAi screening is widely applied in biological research, but remains expensive, infrastructure-intensive and conversion of many assays to HTS applications in microplate format is not feasible.

Results

Here, we describe the optimization of a miniaturized cell spot microarray (CSMA) method, which facilitates utilization of the transfection microarray technique for disparate RNAi analyses. To promote rapid adaptation of the method, the concept has been tested with a panel of 92 adherent cell types, including primary human cells. We demonstrate the method in the systematic screening of 492 GPCR coding genes for impact on growth and survival of cultured human prostate cancer cells.

Conclusions

The CSMA method facilitates reproducible preparation

SUBMITTER: Rantala JK 

PROVIDER: S-EPMC3073923 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature