Cyclin E is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells.

ABSTRACT: Cyclin E supports pre-replication complex (pre-RC) assembly, while cyclin A-associated kinase activates DNA synthesis. We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells. In murine embryonic stem cells, Xenopus embryos and human urothelial cells, cyclin E is recruited to the nuclear matrix as cells differentiate and this can be manipulated in vitro. This suggests that pre-RC assembly becomes spatially restricted as template usage is defined. Furthermore, failure to become restricted may contribute to the plasticity of cancer cells.

SUBMITTER: Munkley J 

PROVIDER: S-EPMC3074132 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature