Combining genetical genomics and bulked segregant analysis-based differential expression: an approach to gene localization.

ABSTRACT: Positional gene isolation in unsequenced species generally requires either a reference genome sequence or an inference of gene content based on conservation of synteny with a genomic model. In the large unsequenced genomes of the Triticeae cereals the latter, i.e. conservation of synteny with the rice and Brachypodium genomes, provides a powerful proxy for establishing local gene content and order. However, efficient exploitation of conservation of synteny requires 'homology bridges' between the model genome and the target region that contains a gene of interest. As effective homology bridges are generally the sequences of genetically mapped genes, increasing the density of these genes around a target locus is an important step in the process. We used bulked segregant analysis (BSA) of tra

SUBMITTER: Chen X 

PROVIDER: S-EPMC3075405 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature