Characterization of Helicobacter pylori factors that control transformation frequency and integration length during inter-strain DNA recombination.

ABSTRACT: Helicobacter pylori is a genetically diverse bacterial species, owing in part to its natural competence for DNA uptake that facilitates recombination between strains. Inter-strain DNA recombination occurs during human infection and the H. pylori genome is in linkage equilibrium worldwide. Despite this high propensity for DNA exchange, little is known about the factors that limit the extent of recombination during natural transformation. Here, we identify restriction-modification (R-M) systems as a barrier to transformation with homeologous DNA and find that R-M systems and several components of the recombination machinery control integration length. Type II R-M systems, the nuclease nucT and resolvase ruvC reduced integration length whereas the helicase recG increased it. In addition, we c

SUBMITTER: Humbert O 

PROVIDER: S-EPMC3075595 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature