A highly parallel method for synthesizing DNA repeats enables the discovery of 'smart' protein polymers.

ABSTRACT: Robust high-throughput synthesis methods are needed to expand the repertoire of repetitive protein-polymers for different applications. To address this need, we developed a new method, overlap extension rolling circle amplification (OERCA), for the highly parallel synthesis of genes encoding repetitive protein-polymers. OERCA involves a single PCR-type reaction for the rolling circle amplification of a circular DNA template and simultaneous overlap extension by thermal cycling. We characterized the variables that control OERCA and demonstrated its superiority over existing methods, its robustness, high-throughput and versatility by synthesizing variants of elastin-like polypeptides (ELPs) and protease-responsive polymers of glucagon-like peptide-1 analogues. Despite the GC-rich, highly rep

SUBMITTER: Amiram M 

PROVIDER: S-EPMC3075872 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature