Separation of the bacterial species, Escherichia coli, from mixed-species microbial communities for transcriptome analysis.

ABSTRACT:

Background

The study of bacterial species interactions in a mixed-species community can be facilitated by transcriptome analysis of one species in the community using cDNA microarray technology. However, current applications of microarrays are mostly limited to single species studies. The purpose of this study is to develop a method to separate one species, Escherichia coli as an example, from mixed-species communities for transcriptome analysis.

Results

E. coli cells were separated from a dual-species (E. coli and Stenotrophomonas maltophilia) community using immuno-magnetic separation (IMS). High recovery rates of E. coli were achieved. The purity of E. coli cells was as high as 95.0% separated from suspended mixtures consisting of 1.1 - 71.3% E. coli, and as high as 96.0%

SUBMITTER: Dai D 

PROVIDER: S-EPMC3076228 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature