A novel signaling pathway mediated by the nuclear targeting of C-terminal fragments of mammalian Patched 1.

ABSTRACT:

Background

Patched 1 (Ptc1) is a polytopic receptor protein that is essential for growth and differentiation. Its extracellular domains accept its ligand, Sonic Hedgehog, while the function of its C-terminal intracellular domain is largely obscure.

Principal findings

In this study, we stably expressed human Ptc1 protein in HeLa cells and found that it is subjected to proteolytic cleavage at the C-terminus, resulting in the generation of soluble C-terminal fragments. These fragments accumulated in the nucleus, while the N-terminal region of Ptc1 remained in the cytoplasmic membrane fractions. Using an anti-Ptc1 C-terminal domain antibody, we provide conclusive evidence that C-terminal fragments of endogenous Ptc1 accumulate in the nucleus of C3H10T1/2 cells. Similar nuclear a

SUBMITTER: Kagawa H 

PROVIDER: S-EPMC3076429 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature