A new trick (hydroxyl radical generation) for an old vitamin (B12).

ABSTRACT: Photolysis of hydroxocobalamin in the presence of plasmid DNA (pBR322) results in DNA cleavage. Temporal control of hydroxyl radical production and DNA strand scission by hydroxocobalamin was demonstrated using a 2-deoxyribose assay and a plasmid relaxation assay, respectively. The light-driven hydroxocobalamin-mediated catalytic formation of hydroxyl radicals was demonstrated using radical scavenging studies of DNA cleavage and via recycling of a hydroxocobalamin-resin conjugate several times without loss of efficacy.

SUBMITTER: Shell TA 

PROVIDER: S-EPMC3077068 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature