Activator-induced dynamic disorder and molecular memory in human two-pore domain hTREK1 K channel.

ABSTRACT:

Unlabelled

Ion channels are fundamental molecules in the nervous system that catalyze the flux of ions across the cell membrane. Ion channel flux activity is comparable to the catalytic activity of enzyme molecules. Saturating concentrations of substrate induce "dynamic disorder" in the kinetic rate processes of single-enzyme molecules and consequently, develop correlative "memory" of the previous history of activities. Similarly, binding of ions as substrate alone or in presence of agonists affects the catalytic turnover of single-ion channels. Here, we investigated the possible existence of dynamic disorder and molecular memory in the single human-TREK1-channel due to binding of substrate/agonist using the excised inside-out patch-clamp technique. Our results suggest that the sin

SUBMITTER: Nayak TK 

PROVIDER: S-EPMC3077215 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature