Chromatin immunoprecipitation to analyze DNA binding sites of HMGA2.

ABSTRACT:

Background

HMGA2 is an architectonic transcription factor abundantly expressed during embryonic and fetal development and it is associated with the progression of malignant tumors. The protein harbours three basically charged DNA binding domains and an acidic protein binding C-terminal domain. DNA binding induces changes of DNA conformation and hence results in global overall change of gene expression patterns. Recently, using a PCR-based SELEX (Systematic Evolution of Ligands by Exponential Enrichment) procedure two consensus sequences for HMGA2 binding have been identified.

Methodology/principal findings

In this investigation chromatin immunoprecipitation (ChIP) experiments and bioinformatic methods were used to analyze if these binding sequences can be verified on chromat

SUBMITTER: Winter N 

PROVIDER: S-EPMC3077414 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature