A rewired green fluorescent protein: folding and function in a nonsequential, noncircular GFP permutant.

ABSTRACT: The sequential order of secondary structural elements in proteins affects the folding and activity to an unknown extent. To test the dependence on sequential connectivity, we reconnected secondary structural elements by their solvent-exposed ends, permuting their sequential order, called "rewiring". This new protein design strategy changes the topology of the backbone without changing the core side chain packing arrangement. While circular and noncircular permutations have been observed in protein structures that are not related by sequence homology, to date no one has attempted to rationally design and construct a protein with a sequence that is noncircularly permuted while conserving three-dimensional structure. Herein, we show that green fluorescent protein can be rewired, still functio

SUBMITTER: Reeder PJ 

PROVIDER: S-EPMC3077829 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature