Requirements for membrane attack complex formation and anaphylatoxins binding to collagen-activated platelets.

ABSTRACT:

Background

The activation of complement during platelet activation is incompletely understood.

Objectives

We sought to explore the formation of C5b-9 and anaphylatoxins binding to collagen-activated platelets.

Methods

C5b-9, anaphylatoxins C3a, C4a and C5a, and anaphylatoxin receptors C3aR1 and C5aR were measured by flow cytometry and/or confocal microscopy. Platelet microparticles were quantified by flow cytometry, and their C5b-9 content was determined by western blot analyses. In all experiments, sodium citrate was used for blood anticoagulation.

Results

C5b-9 rapidly formed on the platelet surface following activation with collagen, TRAP, ADP or A23187, but was surprisingly restricted to a subset of platelets (1 to 15%) independently of P-selectin or phosph

SUBMITTER: Martel C 

PROVIDER: S-EPMC3078139 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature