Glutathione synthesis is essential for pollen germination in vitro.

ABSTRACT:

Background

The antioxidant glutathione fulfills many important roles during plant development, growth and defense in the sporophyte, however the role of this important molecule in the gametophyte generation is largely unclear. Bioinformatic data indicate that critical control enzymes are negligibly transcribed in pollen and sperm cells. Therefore, we decided to investigate the role of glutathione synthesis for pollen germination in vitro in Arabidopsis thaliana accession Col-0 and in the glutathione deficient mutant pad2-1 and link it with glutathione status on the subcellular level.

Results

The depletion of glutathione by buthionine sulfoximine (BSO), an inhibitor of glutathione synthesis, reduced pollen germination rates to 2-5% compared to 71% germination in wildtype cont

SUBMITTER: Zechmann B 

PROVIDER: S-EPMC3078877 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature