Adipose tissue-derived mesenchymal stem cell-based liver gene delivery.

ABSTRACT:

Background & aims

The adipose tissue represents an accessible, abundant, and replenishable source of adult stem cells for potential applications in regenerative medicine. Adipose tissue-derived mesenchymal stem cells (AT-MSCs) resemble bone marrow-derived mesenchymal stem cells (BM-MSCs) with respect to morphology, immune-phenotype, and multiple differentiation capability. In the present study, we investigated the feasibility of AT-MSC-based liver gene delivery for the treatment of alpha 1-antitrypsin deficiency.

Methods

Mouse AT-MSCs were transduced by rAAV vectors and transplanted into the mouse liver.

Results

We showed that AT-MSCs can be transduced by recombinant adeno-associated viral vector serotype 1 (rAAV1-CB-hAAT). After transplanting to the mouse liver, ex v

SUBMITTER: Li H 

PROVIDER: S-EPMC3079008 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature