Denaturing gradient gel electrophoresis of neonatal intestinal microbiota in relation to the development of asthma.

ABSTRACT:

Background

The extended 'hygiene hypothesis' suggests that the initial composition of the infant gut microbiota is a key determinant in the development of atopic disease. Several studies have demonstrated that the microbiota of allergic and non-allergic infants are different even before the development of symptoms, with a critical time window during the first 6 months of life. The aim of the study was to investigate the association between early intestinal colonisation and the development of asthma in the first 3 years of life using DGGE (denaturing gradient gel electrophoresis).

Methods

In a prospective birth cohort, 110 children were classified according to the API (Asthma Predictive Index). A positive index included wheezing during the first three years of life combined w

SUBMITTER: Vael C 

PROVIDER: S-EPMC3079593 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature