Comparison of expression, purification and characterization of a new pectate lyase from Phytophthora capsici using two different methods.

ABSTRACT:

Background

Pectate lyases (PELs) play an important role in the infection process of plant pathogens and also have a commercial significance in industrial applications. Most of the PELs were expressed as soluble recombinant proteins, while a few recombinant proteins were insoluble. The production of a large-scale soluble recombinant PEL would allow not only a more detailed structural and functional characterization of this enzyme but also may have important applications in the food industry.

Results

We cloned a new pectate lyase gene (Pcpel2) from Phytophthora capsici. Pcpel2 was constructed by pET system and pMAL system, and both constructs were used to express the PCPEL2 in Escherichia coli BL21 (DE3) pLysS. The expressed products were purified using affinity chromatography

SUBMITTER: Wang H 

PROVIDER: S-EPMC3079630 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature