Detection and mapping of mtDNA SNPs in Atlantic salmon using high throughput DNA sequencing.

ABSTRACT:

Background

Approximately half of the mitochondrial genome inherent within 546 individual Atlantic salmon (Salmo salar) derived from across the species' North Atlantic range, was selectively amplified with a novel combination of standard PCR and pyro-sequencing in a single run using 454 Titanium FLX technology (Roche, 454 Life Sciences). A unique combination of barcoded primers and a partitioned sequencing plate was employed to designate each sequence read to its original sample. The sequence reads were aligned according to the S. salar mitochondrial reference sequence (NC_001960.1), with the objective of identifying single nucleotide polymorphisms (SNPs). They were validated if they met with the following three stringent criteria: (i) sequence reads were produced from both DNA stra

SUBMITTER: Fridjonsson O 

PROVIDER: S-EPMC3079667 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature