Massive-scale RNA-Seq analysis of non ribosomal transcriptome in human trisomy 21.

ABSTRACT: Hybridization- and tag-based technologies have been successfully used in Down syndrome to identify genes involved in various aspects of the pathogenesis. However, these technologies suffer from several limits and drawbacks and, to date, information about rare, even though relevant, RNA species such as long and small non-coding RNAs, is completely missing. Indeed, none of published works has still described the whole transcriptional landscape of Down syndrome. Although the recent advances in high-throughput RNA sequencing have revealed the complexity of transcriptomes, most of them rely on polyA enrichment protocols, able to detect only a small fraction of total RNA content. On the opposite end, massive-scale RNA sequencing on rRNA-depleted samples allows the survey of the complete set of c

SUBMITTER: Costa V 

PROVIDER: S-EPMC3080369 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature