Microarray analysis of immediate-type allergy in KU812 cells in response to fulvic acid.

ABSTRACT: Fulvic acid (FA) is class of compounds of humic substances formed through the degradation of organic substances by chemical and biological processes. FA has been utilized in traditional Chinese medicine and possesses various pharmacological properties. Previously, we reported that FA extracted from solubilized excess sludge (SS-FA) had an inhibitory effect on β-hexosaminidase release in human leukemia basophilic (KU812) cells. In this study, we investigated the effects of SS-FA on the immediate-type allergic reaction and studied its possible mechanisms of action in KU812 cells following activation with phorbol myristate acetate (20 nmol L(-1)) plus calcium ionophore A23187 (1 μmol L(-1)) (PMACI). The inhibitory effect of SS-FA on degranulation in PMACI-stimulated KU812 cells was examined u

SUBMITTER: Motojima H 

PROVIDER: S-EPMC3080475 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature