Constitutive expression and enzymatic cleavage of ICAM-1 in the spontaneously hypertensive rat.

ABSTRACT:

Background/aims

Leukocyte adhesion to the endothelium is abnormal in hypertension. We have recently shown that spontaneously hypertensive rats (SHRs) have circulating leukocytes with enhanced CD18 receptor cleavage. In the current study, we investigate expression levels of its counter receptor, intercellular adhesion molecule (ICAM-1), and its possible proteolytic cleavage in the SHR and control Wistar rat.

Methods

ICAM-1 was labeled on tissue sections with two antibodies targeting its extracellular and intracellular domains and evaluated by light absorption measurements. The in situ cleavage of ICAM-1 was assessed by treating vessel sections with matrix metalloproteinase (MMP)-7, MMP-9 and elastase.

Results

SHRs showed a significant increase in ICAM-1 expression in l

SUBMITTER: Tong S 

PROVIDER: S-EPMC3080588 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature