Developmental variations in metabolic capacity of flavin-containing mono-oxygenase 3 in childhood.

ABSTRACT:

Aim

The aim of this study was to investigate intra- and inter-individual variations of functional metabolic capacity of flavin-containing mono-oxygenase (FMO) during childhood using trimethylamine N-oxygenation as a probe reaction.

Methods

Trimethylamine N-oxygenation functional activity and presence of FMO1 (fetal form), FMO3 (adult form), and FMO5 (endogenous form) were immunochemically determined and compared in human liver microsomes obtained from children at various ages. As a control, the same parameters were studied with recombinant FMO1, FMO3 and FMO5 proteins as enzyme sources. Developmental variation in functional metabolic capacity of FMO was estimated by measuring urinary trimethylamine and its N-oxide in several individuals at different ages and in a group of 77

SUBMITTER: Shimizu M 

PROVIDER: S-EPMC3080647 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature