Computational prediction and experimental validation of novel markers for detection of STEC O157:H7.

ABSTRACT:

Aim

To identify and assess the novel makers for detection of Shiga toxin producing Escherichia coli (STEC) O157:H7 with an integrated computational and experimental approach.

Methods

High-throughput NCBI blast (E-value cutoff e-5) was used to search homologous genes among all sequenced prokaryotic genomes of each gene encoded in each of the three strains of STEC O157:H7 with complete genomes, aiming to find unique genes in O157:H7 as its potential markers. To ensure that the identified markers from the three strains of STEC O157:H7 can serve as general markers for all the STEC O157:H7 strains, a genomic barcode approach was used to select the markers to minimize the possibility of choosing a marker gene as part of a transposable element. Effectiveness of the markers predicte

SUBMITTER: Wang GQ 

PROVIDER: S-EPMC3080728 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature