Systems analysis of endothelial cell plasma membrane proteome of rat lung microvasculature.

ABSTRACT:

Background

Endothelial cells line all blood vessels to form the blood-tissue interface which is critical for maintaining organ homeostasis and facilitates molecular exchange. We recently used tissue subcellular fractionation combined with several multi-dimensional mass spectrometry-based techniques to enhance identification of lipid-embedded proteins for large-scale proteomic mapping of luminal endothelial cell plasma membranes isolated directly from rat lungs in vivo. The biological processes and functions of the proteins expressed at this important blood-tissue interface remain unexplored at a large scale.

Results

We performed an unbiased systems analysis of the endothelial cell surface proteome containing over 1800 proteins to unravel the major functions and pathways appa

SUBMITTER: Li Y 

PROVIDER: S-EPMC3080792 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature