Epigenetic regulation of cell type-specific expression patterns in the human mammary epithelium.

ABSTRACT: Differentiation is an epigenetic program that involves the gradual loss of pluripotency and acquisition of cell type-specific features. Understanding these processes requires genome-wide analysis of epigenetic and gene expression profiles, which have been challenging in primary tissue samples due to limited numbers of cells available. Here we describe the application of high-throughput sequencing technology for profiling histone and DNA methylation, as well as gene expression patterns of normal human mammary progenitor-enriched and luminal lineage-committed cells. We observed significant differences in histone H3 lysine 27 tri-methylation (H3K27me3) enrichment and DNA methylation of genes expressed in a cell type-specific manner, suggesting their regulation by epigenetic mechanisms and a d

SUBMITTER: Maruyama R 

PROVIDER: S-EPMC3080862 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature