C-Crk proto-oncogene contributes to transcriptional repression of p120-catenin in non-small cell lung cancer cells.

ABSTRACT: As a member of adherens junction, p120-catenin (p120ctn) plays a major role in cell adhesions through stabilization of E-cadherin. p120ctn is transcriptionally down-regulated in non-small cell lung cancer (NSCLC), although the molecular mechanisms underlying p120ctn repression are incompletely defined. Here we further investigated transcriptional regulation of p120ctn in NSCLC. We prepared a promoter reporter plasmid construct that contained p120ctn promoter region from position -1082 to +320 relative to transcription start site. Through serial deletion mutation analysis of the p120ctn promoter, we pinpointed cis-acting elements involved in regulation of p120ctn. We identified transcription factor SP1 as a transcriptional repressor of p120ctn that directly binds to segment (-9 to +36) of t

SUBMITTER: Mortazavi F 

PROVIDER: S-EPMC3081060 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature