Ectopic overexpression of haem oxygenase-1 protects kidneys from carboplatin-mediated apoptosis.

ABSTRACT:

Background and purpose

We previously reported that the activation of the nuclear factor of activated T-lymphocyte-3 (NFAT3) by carboplatin leads to renal apoptosis as a result of oxidative stress, which is reversed by N-acetylcysteine. Herein, we extend our previous work to provide evidence of the molecular mechanisms of haem oxygenase (HO)-1 in protecting against injury.

Experimental approach

Protective mechanisms of HO-1 in carboplatin-mediated renal apoptosis were examined in C57BL/6 mice and rat renal tubular cells (RTC) with HO-1 induction or inactivation/knockdown.

Key results

The HO-1, induced by cobalt protoporphyrin, protected against carboplatin-induced renal injury in vivo. This protection was decreased by an inhibitor of HO-1 action, tin protoporphyrin. In

SUBMITTER: Sue YM 

PROVIDER: S-EPMC3081116 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature