KIR receptor-ligand incompatibility predicts killing of osteosarcoma cell lines by allogeneic NK cells.

ABSTRACT:

Background

The effectiveness of killer immunoglobulin-like receptor (KIR) incompatible, alloreactive natural killer (NK) cells has been primarily documented in hematological malignancies following stem-cell transplant. This effect has not been thoroughly evaluated for pediatric solid tumors. In this study, we evaluated KIR receptor-ligand incompatibility of NK cells against osteosarcoma cell lines.

Procedure

Following the KIR receptor-ligand mismatch model, MHC I cell surface expression and KIR ligand mRNA content of 3 osteosarcoma cell lines was determined by flow cytometry and quantitative reverse transcription-polymerase chain reaction (qRT-PCR), respectively. NK cells were isolated from healthy volunteer donor peripheral blood mononuclear cells (PBMCs) and KIR surface ex

SUBMITTER: Delgado D 

PROVIDER: S-EPMC3081247 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature