Methods for the Analysis of High Precision Differential Hydrogen Deuterium Exchange Data.

ABSTRACT: Hydrogen/deuterium exchange (HDX) mass spectrometry has been widely applied to the characterization of protein dynamics. More recently, differential HDX has been shown to be effective for the characterization of ligand binding. Previously we have described a fully automated HDX system for use as a ligand screening platform. Here we describe and validate the required data analysis workflow to facilitate the use of HDX as a robust approach for ligand screening. Following acquisition of HDX data at a single on-exchange time point (n ≥ 3), one way analysis of variance in conjunction with the Tukey multiple comparison procedure is used to establish the significance of any measured difference. Analysis results are graphed with respect to a single peptide, ligand or group of ligands, or displayed

SUBMITTER: Chalmers MJ 

PROVIDER: S-EPMC3081588 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature