A combined global and local approach to elucidate spatial organization of the Mycobacterial ParB-parS partition assembly.

ABSTRACT: Combining diverse sets of data at global (size, shape) and local (residue) scales is an emerging trend for elucidating the organization and function of the cellular assemblies. We used such a strategy, combining data from X-ray and neutron scattering with H/D-contrast variation and X-ray footprinting with mass spectrometry, to elucidate the spatial organization of the ParB-parS assembly from Mycobacterium tuberculosis. The ParB-parS participates in plasmid and chromosome segregation and condensation in predivisional bacterial cells. ParB polymerizes around the parS centromere(s) to form a higher-order assembly that serves to recruit cyto-skeletal ParA ATPases and SMC proteins for chromosome segregation. A hybrid model of the ParB-parS was built by combining and correlating computational mo

SUBMITTER: Chaudhuri BN 

PROVIDER: S-EPMC3081668 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature