Stochastic variation of transcript abundance in C57BL/6J mice.

ABSTRACT:

Background

Transcripts can exhibit significant variation in tissue samples from inbred laboratory mice. We have designed and carried out a microarray experiment to examine transcript variation across samples from adipose, heart, kidney, and liver tissues of C57BL/6J mice and to partition variation into within-mouse and between-mouse components. Within-mouse variance captures variation due to heterogeneity of gene expression within tissues, RNA-extraction, and array processing. Between-mouse variance reflects differences in transcript abundance between genetically identical mice.

Results

The nature and extent of transcript variation differs across tissues. Adipose has the largest total variance and the largest within-mouse variance. Liver has the smallest total variance, but

SUBMITTER: Vedell PT 

PROVIDER: S-EPMC3082245 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature