An electrochemical supersandwich assay for sensitive and selective DNA detection in complex matrices.

ABSTRACT: In a traditional sandwich assay, a DNA target hybridizes to a single copy of the signal probe. Here we employ a modified signal probe containing a methylene blue (a redox moiety) label and a "sticky end." When a DNA target hybridizes this signal probe, the sticky end remains free to hybridize another target leading to the creation of a supersandwich structure containing multiple labels. This leads to large signal amplification upon monitoring by voltammetry.

SUBMITTER: Xia F 

PROVIDER: S-EPMC3082473 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature