Functional rundown of gamma-aminobutyric acid(A) receptors in human hypothalamic hamartomas.

ABSTRACT:

Objective

Human hypothalamic hamartomas (HHs) are highly associated with treatment-resistant gelastic seizures. HHs are intrinsically epileptogenic, although the basic cellular mechanisms responsible for seizure activity are unknown. Altered gamma-aminobutyric acid (GABA) function can contribute to epileptogenesis in humans and animal models. Recently, functional GABA(A) receptor (GABA(A) R) rundown has been described in surgically resected human temporal lobe epilepsy tissue. We asked whether functional GABA(A) R rundown also occurs in human HH neurons.

Methods

GABA(A) R-mediated currents were measured using perforated patch-clamp recordings in single neurons acutely dissociated from surgically resected HH tissue. In addition, functional GABA(A) Rs were expressed in Xenopus

SUBMITTER: Li G 

PROVIDER: S-EPMC3082609 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature