Genome-wide evidence for an essential role of the human Staf/ZNF143 transcription factor in bidirectional transcription.

ABSTRACT: In the human genome, ∼ 10% of the genes are arranged head to head so that their transcription start sites reside within <1 kbp on opposite strands. In this configuration, a bidirectional promoter generally drives expression of the two genes. How bidirectional expression is performed from these particular promoters constitutes a puzzling question. Here, by a combination of in silico and biochemical approaches, we demonstrate that hStaf/ZNF143 is involved in controlling expression from a subset of divergent gene pairs. The binding sites for hStaf/ZNF143 (SBS) are overrepresented in bidirectional versus unidirectional promoters. Chromatin immunoprecipitation assays with a significant set of bidirectional promoters containing putative SBS revealed that 93% of them are associated with hStaf/ZNF

SUBMITTER: Anno YN 

PROVIDER: S-EPMC3082894 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature