Diverse gene reprogramming events occur in the same spatial clusters of distal regulatory elements.

ABSTRACT: The spatial organization of genes in the interphase nucleus plays an important role in establishment and regulation of gene expression. Contradicting results have been reported to date, with little consensus about the dynamics of nuclear organization and the features of the contact loci. In this study, we investigated the properties and dynamics of genomic loci that are in contact with glucocorticoid receptor (GR)-responsive loci. We took a systematic approach, combining genome-wide interaction profiling by the chromosome conformation capture on chip (4C) technology with expression, protein occupancy, and chromatin accessibility profiles. This approach allowed a comprehensive analysis of how distinct features of the linear genome are organized in the three-dimensional nuclear space in the

SUBMITTER: Hakim O 

PROVIDER: S-EPMC3083086 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature