CGMP signals modulate cAMP levels in a compartment-specific manner to regulate catecholamine-dependent signaling in cardiac myocytes.

ABSTRACT:

Rationale

cAMP and cGMP are intracellular second messengers involved in heart pathophysiology. cGMP can potentially affect cAMP signals via cGMP-regulated phosphodiesterases (PDEs).

Objective

To study the effect of cGMP signals on the local cAMP response to catecholamines in specific subcellular compartments.

Methods and results

We used real-time FRET imaging of living rat ventriculocytes expressing targeted cAMP and cGMP biosensors to detect cyclic nucleotides levels in specific locales. We found that the compartmentalized, but not the global, cAMP response to isoproterenol is profoundly affected by cGMP signals. The effect of cGMP is to increase cAMP levels in the compartment where the protein kinase (PK)A-RI isoforms reside but to decrease cAMP in the compartment w

SUBMITTER: Stangherlin A 

PROVIDER: S-EPMC3083836 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature