Immobilization and one-dimensional arrangement of virus capsids with nanoscale precision using DNA origami.

ABSTRACT: DNA origami was used as a scaffold to arrange spherical virus capsids into one-dimensional arrays with precise nanoscale positioning. To do this, we first modified the interior surface of bacteriophage MS2 capsids with fluorescent dyes as a model cargo. An unnatural amino acid on the external surface was then coupled to DNA strands that were complementary to those extending from origami tiles. Two different geometries of DNA tiles (rectangular and triangular) were used. The capsids associated with tiles of both geometries with virtually 100% efficiency under mild annealing conditions, and the location of capsid immobilization on the tile could be controlled by the position of the probe strands. The rectangular tiles and capsids could then be arranged into one-dimensional arrays by adding D

SUBMITTER: Stephanopoulos N 

PROVIDER: S-EPMC3083853 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature