Chemometrics of differentially expressed proteins from colorectal cancer patients.

ABSTRACT:

Aim

To evaluate the usefulness of differentially expressed proteins from colorectal cancer (CRC) tissues for differentiating cancer and normal tissues.

Methods

A Proteomic approach was used to identify the differentially expressed proteins between CRC and normal tissues. The proteins were extracted using Tris buffer and thiourea lysis buffer (TLB) for extraction of aqueous soluble and membrane-associated proteins, respectively. Chemometrics, namely principal component analysis (PCA) and linear discriminant analysis (LDA), were used to assess the usefulness of these proteins for identifying the cancerous state of tissues.

Results

Differentially expressed proteins identified were 37 aqueous soluble proteins in Tris extracts and 24 membrane-associated proteins in TLB ext

SUBMITTER: Yeoh LC 

PROVIDER: S-EPMC3084394 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature