Intraperoxisomal redox balance in mammalian cells: oxidative stress and interorganellar cross-talk.

ABSTRACT: Reactive oxygen species (ROS) are at once unsought by-products of metabolism and critical regulators of multiple intracellular signaling cascades. In nonphotosynthetic eukaryotic cells, mitochondria are well-investigated major sites of ROS generation and related signal initiation. Peroxisomes are also capable of ROS generation, but their contribution to cellular oxidation-reduction (redox) balance and signaling events are far less well understood. In this study, we use a redox-sensitive variant of enhanced green fluorescent protein (roGFP2-PTS1) to monitor the state of the peroxisomal matrix in mammalian cells. We show that intraperoxisomal redox status is strongly influenced by environmental growth conditions. Furthermore, disturbances in peroxisomal redox balance, although not necessaril

SUBMITTER: Ivashchenko O 

PROVIDER: S-EPMC3084667 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature