Comparison of three targeted enrichment strategies on the SOLiD sequencing platform.

ABSTRACT: Despite the ever-increasing throughput and steadily decreasing cost of next generation sequencing (NGS), whole genome sequencing of humans is still not a viable option for the majority of genetics laboratories. This is particularly true in the case of complex disease studies, where large sample sets are often required to achieve adequate statistical power. To fully leverage the potential of NGS technology on large sample sets, several methods have been developed to selectively enrich for regions of interest. Enrichment reduces both monetary and computational costs compared to whole genome sequencing, while allowing researchers to take advantage of NGS throughput. Several targeted enrichment approaches are currently available, including molecular inversion probe ligation sequencing (MIPS),

SUBMITTER: Hedges DJ 

PROVIDER: S-EPMC3084696 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature