Mamld1 knockdown reduces testosterone production and Cyp17a1 expression in mouse Leydig tumor cells.

ABSTRACT:

Background

MAMLD1 is known to be a causative gene for hypospadias. Although previous studies have indicated that MAMLD1 mutations result in hypospadias primarily because of compromised testosterone production around the critical period for fetal sex development, the underlying mechanism(s) remains to be clarified. Furthermore, although functional studies have indicated a transactivation function of MAMLD1 for the non-canonical Notch target Hes3, its relevance to testosterone production remains unknown. To examine these matters, we performed Mamld1 knockdown experiments.

Methodology/principal findings

Mamld1 knockdown was performed with two siRNAs, using mouse Leydig tumor cells (MLTCs). Mamld1 knockdown did not influence the concentrations of pregnenolone and progesterone bu

SUBMITTER: Nakamura M 

PROVIDER: S-EPMC3084764 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature