Regulatory feedback loop of two phz gene clusters through 5'-untranslated regions in Pseudomonas sp. M18.

ABSTRACT:

Background

Phenazines are important compounds produced by pseudomonads and other bacteria. Two phz gene clusters called phzA1-G1 and phzA2-G2, respectively, were found in the genome of Pseudomonas sp. M18, an effective biocontrol agent, which is highly homologous to the opportunistic human pathogen P. aeruginosa PAO1, however little is known about the correlation between the expressions of two phz gene clusters.

Methodology/principal findings

Two chromosomal insertion inactivated mutants for the two gene clusters were constructed respectively and the correlation between the expressions of two phz gene clusters was investigated in strain M18. Phenazine-1-carboxylic acid (PCA) molecules produced from phzA2-G2 gene cluster are able to auto-regulate expression itself and activat

SUBMITTER: Li Y 

PROVIDER: S-EPMC3084852 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature