A parallel microfluidic flow cytometer for high-content screening.

ABSTRACT: A parallel microfluidic cytometer (PMC) uses a high-speed scanning photomultiplier-based detector to combine low-pixel-count, one-dimensional imaging with flow cytometry. The 384 parallel flow channels of the PMC decouple count rate from signal-to-noise ratio. Using six-pixel one-dimensional images, we investigated protein localization in a yeast model for human protein misfolding diseases and demonstrated the feasibility of a nuclear-translocation assay in Chinese hamster ovary (CHO) cells expressing an NF?B-EGFP reporter.

SUBMITTER: McKenna BK 

PROVIDER: S-EPMC3084896 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature