Chemical noise reduction via mass spectrometry and ion/ion charge inversion: amino acids.

ABSTRACT: Charge inversion ion/ion reactions can provide a significant reduction in chemical noise associated with mass spectra derived from complex mixtures for species composed of both acidic and basic sites, provided the ions derived from the matrix largely undergo neutralization. Amino acids constitute an important class of amphoteric compounds that undergo relatively efficient charge inversion. Precipitated plasma constitutes a relatively complex biological matrix that yields detectable signals at essentially every mass-to-charge value over a wide range. This chemical noise can be dramatically reduced using multiply charged reagent ions that can invert the charge of species amenable to the transfer of multiple charges upon a single interaction and by detecting product ions of opposite polarity.

SUBMITTER: Hassell KM 

PROVIDER: S-EPMC3084898 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature