Ontology highlight
ABSTRACT:
SUBMITTER: He N
PROVIDER: S-EPMC3085314 | biostudies-literature | 2010 May
REPOSITORIES: biostudies-literature
Molecular cell 20100501 3
Recruitment of the P-TEFb kinase by HIV-1 Tat to the viral promoter triggers the phosphorylation and escape of RNA polymerase II from promoter-proximal pausing. It is unclear, however, if Tat recruits additional host factors that further stimulate HIV-1 transcription. Using a sequential affinity-purification scheme, we have identified human transcription factors/coactivators AFF4, ENL, AF9, and elongation factor ELL2 as components of the Tat-P-TEFb complex. Through the bridging functions of Tat ...[more]