A qualitative and quantitative proteomic study of human microdialysate and the cutaneous response to injury.

ABSTRACT: The extracellular fluid space is the site of intercellular communication and represents an important source of mediators that can shed light on the parenchymal environment. Sampling of this compartment using continuous microdialysis allows assessment of the temporal changes in extracellular mediators involved in tissue homeostasis and disease processes. However, novel biomarker identification is limited by the current need to utilize specific, targeted molecular assays. The aim of our study was to explore the use of qualitative and quantitative proteomic approaches to define the protein content of dermal dialysate. Timed dermal dialysate samples were collected from healthy human volunteers for 5 h following probe insertion, using a 3,000-kDa MWCO membrane perfused at a rate of 3 μl/min. Di

SUBMITTER: Gill C 

PROVIDER: S-EPMC3085710 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature