A Dictyostelium SH2 adaptor protein required for correct DIF-1 signaling and pattern formation.

ABSTRACT: Dictyostelium is the only non-metazoan with functionally analyzed SH2 domains and studying them can give insights into their evolution and wider potential. LrrB has a novel domain configuration with leucine-rich repeat, 14-3-3 and SH2 protein-protein interaction modules. It is required for the correct expression of several specific genes in early development and here we characterize its role in later, multicellular development. During development in the light, slug formation in LrrB null (lrrB-) mutants is delayed relative to the parental strain, and the slugs are highly defective in phototaxis and thermotaxis. In the dark the mutant arrests development as an elongated mound, in a hitherto unreported process we term dark stalling. The developmental and phototaxis defects are cell autonomou

SUBMITTER: Sugden C 

PROVIDER: S-EPMC3085826 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature