Analysis of the human pancreatic stellate cell secreted proteome.

ABSTRACT:

Objective

Pancreatic stellate cells (PSCs) are important players in pancreatic fibrosis and are major contributors to the extracellular matrix proteins observed with the stromal response characteristic of pancreatic ductal adenocarcinoma (PDAC). Pancreatic stellate cells are also believed to secrete soluble factors that promote tumor progression; however, no comprehensive analysis of the PSC proteome in either the quiescent or the activated state has been reported.

Methods

Using 2-dimensional tandem mass spectrometry and the RLT-PSC cell line, we present the first comprehensive study describing and comparing the quiescent and activated human PSC-secreted proteomes.

Results

Very few proteins are secreted in the quiescent state. In stark contrast, activated PSCs secrete

SUBMITTER: Wehr AY 

PROVIDER: S-EPMC3086313 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature