Segmental allergen challenge alters multimeric structure and function of surfactant protein D in humans.

ABSTRACT:

Rationale

Surfactant protein D (SP-D), a 43-kD collectin, is synthesized and secreted by airway epithelia as a dodecamer formed by assembly of four trimeric subunits. We have previously shown that the quaternary structure of SP-D can be altered during inflammatory lung injury through its modification by S-nitrosylation, which in turn alters its functional behavior producing a proinflammatory response in effector cells.

Objectives

We hypothesized that alterations in structure and function of SP-D may occur in humans with acute allergic inflammation.

Methods

Bronchoalveolar lavage (BAL) fluid was collected from 15 nonsmoking patients with mild intermittent allergic asthma before and 24 hours after segmental provocation with saline, allergen, LPS, and mixtures of allerge

SUBMITTER: Atochina-Vasserman EN 

PROVIDER: S-EPMC3086753 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature