The role of matrix metalloproteinase-9 in cigarette smoke-induced emphysema.

ABSTRACT:

Rationale

Matrix metalloprotease (MMP)-9 is an elastolytic endopeptidase produced by activated macrophages that may be involved in the development of human pulmonary emphysema and could be inhibited with existing compounds. Mouse models have demonstrated that excess MMP-9 production can result in permanent alveolar destruction.

Objectives

To determine if MMP-9 causes cigarette smoke-induced emphysema using MMP-9 knockout mice and human samples.

Methods

Mouse lungs were analyzed for inflammation and airspace enlargement using a mainstream smoke-exposure model. Human macrophage mRNA was isolated from subjects with emphysema by laser capture microdissection. Human blood monocyte mRNA was isolated from subjects with greater than 30 pack-year smoking history. Human gene ex

SUBMITTER: Atkinson JJ 

PROVIDER: S-EPMC3086754 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature