Microarray-based analysis of differential gene expression between infective and noninfective larvae of Strongyloides stercoralis.

ABSTRACT:

Background

Differences between noninfective first-stage (L1) and infective third-stage (L3i) larvae of parasitic nematode Strongyloides stercoralis at the molecular level are relatively uncharacterized. DNA microarrays were developed and utilized for this purpose.

Methods and findings

Oligonucleotide hybridization probes for the array were designed to bind 3,571 putative mRNA transcripts predicted by analysis of 11,335 expressed sequence tags (ESTs) obtained as part of the Nematode EST project. RNA obtained from S. stercoralis L3i and L1 was co-hybridized to each array after labeling the individual samples with different fluorescent tags. Bioinformatic predictions of gene function were developed using a novel cDNA Annotation System software. We identified 935 differentially

SUBMITTER: Ramanathan R 

PROVIDER: S-EPMC3086827 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature